Signalling

Part:BBa_M36776:Design

Designed by: Sylvia Yang   Group: Stanford BIOE44 - S11   (2013-12-03)


pH sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A) the sigma factor may change the normal cell behavior. B) low pH would kill cells.


Source

The promoter, GadA sequence comes from 2010 Wisconsin iGem team, the BCD13 sequence from paper "Precise and reliable gene expression via standard transcription and translation initiation elements", the Rpos sequence from "Factor RpoD - Escherichia Coli (strain K12)".

References